Anti-Mouse RORgt

Biology
Abbvie
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies by Thermo Fisher Scientific

Product Name:

anti-mouse RORgt PE

Catalog Number:

12-6981-82

The purpose of this antibody was to use it to quantify the frequency of a certain T cell subset in the naïve spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

0.125ug per test in permeabilization buffer at 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The results show that the entire population of T cells shifted in terms of RORgt expression, which is not what I expected since only a small frequency of cells should express it. A replicate experiment showed no shift. The antibody does not stain bright enough.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use

The Bad

Does not stain bright

The Bottom Line

Try another clone

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