Anti-Mouse CD40

May 01, 2017

Biology
Abbvie
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Bioscience

Product Name:

anti-mouse CD40 BV650

Catalog Number:

740492

The general aim is to determine what the basal level of CD40 is in the BALB/c spleen. The product was selected based on availability on this fluorochrome.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1ug per test, 30min on ice

Blocking Agent

Fc block 5 minutes on ice

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The results show very low expression of CD40 on myeloid cells, which is expected for a naïve animal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Not bright

The Bottom Line

Good antibody to use to detect CD40

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