Ki67 Antibody Works Great For Rat Tissue

pediatrics
University of Mississippi Medical Center
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Ki67 antibody (Rabbit)

Catalog Number:

AB9260

Browse Similar Products: Ki67 Immunohistochemistry Antibodies

The general aim of the research is to study postnatal neurogenesis in animals with maternal immune activation. The specific goals of experiment for which we used the product is to quantify proliferating neural progenitor cells in the subventricular zone of postnatal rats. We have been using this product for more than 10 years, and it is the best compared to several other ki67 antibodies from other companies. 

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Free-floating sections of rat brain

Primary Incubation

Anti-Ki67 at 1:600

Blocking Agent

10% goat serum with 0.5% triton in PBS

Secondary Incubation

Rb-IgG-Alexa 555 (1:1000), RT, 2 h

Tertiary Incubation

None

Detection

Red fluorescence Alexa 555

Results Summary

Works best if the sections are pre-treated with 0.8% triton in PBS for 1 h before applying blocking agents.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright staining with low background. Consistent results.

The Bad

None

The Bottom Line

Best ki67 antibody, works very consistently.

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