Work-horse Instrument

Mitchell Cancer Institute
Mass Spectrometry
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Thermo Orbitrap LTQ-XL

Catalog Number:

IQLAAEGAAPFADBMAOK

This instrument is capable of handling many different sample types from glycans, protein, lipids and small molecules. Our lab has 3 mass spectrometers and while this is not the newest one, it's definitely the one I prefer to use. The data is reproducible and robust. The user interface is a bit tricky but is teachable. Would highly recommend.

Experimental Design and Results Summary

Application

Glycoproteomics

Starting Material

Biofluids (serum and tissue extracts)

Protocol Overview

Samples for this instrument are minimally processed. Major steps include C2 reverse phase chromatography, tripsin digest, followed by offline c18 peptide clean-up standard for most sample types.

Tips

Only inject clean samples, calibrate often

Results Summary

We find this product to be very useful for the many varied areas of research that happen in our lab at any given time. The instrument like any other mass spectrometer needs to be cared for such as routine calibration and preventive maintenance. That aside, it is a very user-friendly instrument, very reliable and for us has lasted many many years.

Features Summary

Fast and robust

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

User-friendly

The Bad

Takes a little time to learn

The Bottom Line

I really like this instrument and it's useful for many applications my research.

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