Dako (Agilent Technologies)
Polyclonal Guinea Pig Anti-Insulin
A056401-2
Browse Similar Products: Insulin Immunofluorescence Antibodies
The focus of my research is to develop a functional immuno-isolating device to protect stem cell-derived beta cells upon transplantation. For this reason we used histology to characterize insulin-producing cells at various stages after transplant. We used this product because of previous successful experience in my previous research projects.
Immunofluorescence
Mouse embryonic pancreata transplanted under the kidney capsule
1/100, over night, 4C
Universal blocking reagent, HK085-5k, Protein block, HK112-9k
Alexa Fluor 568 goat anti-guinea pig IgG, A11075, 1/200, room temperature, 2 hours.
N/A
Confocal microscopy
The histology revealed the time course of embryonic pancreata into mature beta cells.
26982952
Also stained in the picture: Dapi (blue), and Glucagone (green)
Very reliable antibody
Make sure to wash well to avoid background staining
Highly recommended!