DAKO by Agilent Technologies
Polyclonal Rabbit Anti-Human CD3
A045229-2
Browse Similar Products: CD3 Immunohistochemistry Antibodies
We wanted to determine whether T-cells were present in specific sections of the mouse brain during EAE. We used this antibody for Immunohistochemical analysis of 4% paraformaldehyde fixed brain and spinal cord sections
Immunohistochemistry
Mouse Brain and Spinal Cord
Dilution (1:100) overnight at 4 degrees celcius
5% goat serum in 5% milk
Anti-rabbit (1:500)) for 1 hour at room temperature
None
DAB
We were able to detect CD3 positive cells in the dorsal region of the spinal cord and brain
N/A
T-cell specific
Expensive and high volume required
Great antibody to visualize the cellular location of T-cells by IHC