Anti-Human CD4 In Cynomolgus Macaque

MMG
University of Pittsburgh
Graduate Student Researcher

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Quality of Results

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Company:

Abcam

Product Name:

Anti-CD4 antibody [EPR6855]

Catalog Number:

ab133616

We aimed to find a CD4 antibody that would properly stain Cynomolgus macaque formalin fixed-paraffin embedded (IHC-P) tissue sections.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Cynomolgus Macaque Inguinal Lymph Node

Primary Incubation

1:100 at RT for 1hr in humidified chamber

Blocking Agent

0.1% BSA in 1XPBS at RT for 30 min prior to primary incubation in humidified chamber

Secondary Incubation

1:1000 at RT for 1 hr in humidified chamber

Tertiary Incubation

N/A

Detection

Fluorescently labeled secondary antibody (AF488)

Results Summary

Very nice staining in LN sections of cynomolgus macaque tissue

DOI or PMID #

Unpublished

Additional Notes

Tested at 1:100, 1:250, and 1:500. For confocal imaging 1:250 seemed an appropriate dilution

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Summary

The Good

Good staining of CD4 in macaque tissue

The Bad

Price, but comes in a smaller "sample" size as well

The Bottom Line

I would recommend this product for cynomolgus macaque tissues for specific CD4 staining

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