Thermo Scientific
Compact Digital Mini Rotator
88880025
This product is frequently used in our lab for incubation and/or washing of membranes for Western Blots. It was chosen because it is quiet, compact, and has a good range of rotation speeds.
Western blots
PVDF membrane in plastic box
For incubation of membrane with antibody, set speed to 50-60 rpm. Can be done overnight at 4 degrees or for 1.5 - 2 hours at room temperature. For washes, set speed to 100 rpm.
Don't use flasks or tall containers at high speeds without something to hold them.
We achieve cleaner Western blot results, i.e. less background, when membranes are washed thoroughly and when the membrane is rocked or rotated during incubation of the antibodies. This piece of equipment enables that.
Small enough to be placed at 4 degrees, if needed, and very quiet.
N/A
Compact, quiet shaker useful for Western Blot washes
None
Useful piece of equipment for Western Blots and other experiments that require washes or agitation of liquids.