Secondary Anti-Mouse IgG2a APC

Biology
Abbvie
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse IgG2a

Catalog Number:

407109

This antibody was used to detect a primary antibody. This fluorochrome was selected because of its brightness on the cytometer. The cell type used was primary mouse cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary mouse lymphocytes

Primary Incubation

30 minutes on ice

Blocking Agent

Fc block for 10 minutes on ice

Secondary Incubation

0.5ug per test, 20 minutes on ice

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

The results suggest that this secondary antibody can pick up the primary antibody very well. The staining is also very bright.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright

The Bad

High concentration required

The Bottom Line

This is a great antibody for detecting primary antibodies with the mouse IgG2a isotype.

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