IL-4 To Differentiate Bone Marrow Cells

Abbvie
Biology
Scientist

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Quality of Results

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Company:

Peprotech

Product Name:

Recombinant Murine IL-4

Catalog Number:

214-14

The aim of the research was to use cytokines to differentiate bone marrow cells into dendritic cells. This product was chosen because the company is reliable.

Experimental Design and Results Summary

Application

Cell differentiation

Starting Material

Mouse Bone Marrow Cells

Protocol Overview

Cells were incubated with 10ng/ml IL-4 and 10ng/ml GM CSF for 7 days.

Tips

None

Results Summary

The results suggest a homogeneous population for CD11c staining, although all cells express the CD11b marker. This cytokine cocktail worked for my purpose.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Facilitated the differentation

The Bad

May need to be optimized

The Bottom Line

This cytokine facilitated the differentiation of bone marrow cells into dendritic cells.

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