BD Biosciences
Anti-mouse TLR2 BV421
565908
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The general aim of the research is to quantify the levels of TLR2 on dendritic cells in the spleen of naïve mice. This antibody was one of a few clones available commercially.
Flow Cytometry
Mouse splenocytes
5ug/ml for 30 minutes on ice
Fc block for 10minutes on ice
None
Via flow cytometry
This antibody was able to be titrated on mouse splenocytes. The staining is bright. A high concentration had to be used to obtain this bright signal.
N/A
Bright
High concentration required
Other clones for TLR2 may be better at getting brighter signals.