EasySep™ Biotin Positive Selection Kit Review

We routinely purify different cell types for in vitro killing assays where effector cells are tested against HIV infected target cells. We are expressing different TCR vectors in human CD8 T cells to reprogram their recognition of MHC I - peptide on infected cells. These vectors have a mouse CD8 reporter to distinguish the expressed TCR from the endogenous and we use a two step labeling, first with mouse CD8 antibody, then with biotin beads, to purify the transduced cells