Streptavidin Beads

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Streptavidin MicroBeads

Catalog Number:

130-048-102

Our lab performs many types of cell purifications from human and mouse cells for in vitro stimulation assays. Some purifications, such as depletion of all lineage committed cells, require an initial incubation with biotin-conjugated antibodies against many different cell markers. We use these beads as a second step for incubation and then separation of unlabelled cells.

Experimental Design and Results Summary

Application

Cell depletion/ purification

Starting Material

Mouse tissues, or human PBMC

Protocol Overview

Just follow the instructions that come with the product

Tips

Nothing particular, very straightforward

Results Summary

Works well

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Great solution when you need to deplete many cell types expressing different markers

The Bad

None

The Bottom Line

I recommend it.

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