CD14 Microbeads Review

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD14 MicroBeads, human

Catalog Number:

130-050-201

Our laboratory investigates different ways to improve immune responses against HIV, including enhancement of the capacity of effector cells to kill HIV-infected cells. We are currently investigating the capacity of an engineered antibody to trigger ADCC activity in NK cells and monocytes in in vitro assays against an HIV-infected cell line. We use CD14 beads for purification of monocytes and macrophages.

Experimental Design and Results Summary

Application

CD14 cell purification from PBMC

Starting Material

Whole human PBMC

Protocol Overview

Follow the kit protocol, no special adjustments need to be made.

Tips

None

Results Summary

Good separation ~95% purity

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good results

The Bad

None

The Bottom Line

Works well

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