Human CD19 Separation Beads

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD19 MicroBeads, human

Catalog Number:

130-050-301

We are interested in antibody responses that protect against HIV infection, and engineering B cells to express bNAb. We use a humanized mouse model consisting of activated PBMC injected into NSG mouse spleens. We have been setting up experiments where we transduce B cells with lentiviral vectors expressing different bNAb vectors and injected them into the mice to investigate their protective capacity. We purify B cells using these beads.

Experimental Design and Results Summary

Application

B cell purification from PBMC

Starting Material

Whole human PBMC

Protocol Overview

Follow the kit instructions to the details for cell ratios and incubation times.

Tips

Stick to the instructions

Results Summary

Works very well

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works great, very straightforward

The Bad

None

The Bottom Line

I recommend it.

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