Miltenyi Biotec
CD19 MicroBeads, human
130-050-301
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We are interested in antibody responses that protect against HIV infection, and engineering B cells to express bNAb. We use a humanized mouse model consisting of activated PBMC injected into NSG mouse spleens. We have been setting up experiments where we transduce B cells with lentiviral vectors expressing different bNAb vectors and injected them into the mice to investigate their protective capacity. We purify B cells using these beads.
B cell purification from PBMC
Whole human PBMC
Follow the kit instructions to the details for cell ratios and incubation times.
Stick to the instructions
Works very well
N/A
Works great, very straightforward
None
I recommend it.