PBL Assay Science IFN-b alpha

The Wistar Institute
Montaner Lab
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

PBL Assay Science

Product Name:

Human Interferon Beta 1a 1x105

Catalog Number:

11415-1

Our lab has used PBL's IFN-b alpha for a couple months now and have had satisfactory results. Item is processed and shipped quickly on dry ice. The only complaint we would have is if they had a greater concentration available, such as a 1x106 vial.

Experimental Design and Results Summary

Application

Stimulatory reagent in growth media

Starting Material

Fresh human peripheral blood mononuclear cells isolated from a patient the day of the experiment/p>

Protocol Overview

Reagent was added to growth medium and incubated overnight at 37 degrees Celsius 5% CO2. Media was changed daily for 3 days, with 5,000 units of IFN-b added to the media each day.

Tips

Aliquot into working vials prior to use.

Results Summary

IFN-b added in large quantities to stimulated lymphocytes gave us the predicted result of decreasing the amount of proliferating T cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to work with and functions properly.

The Bad

Small concentration for our usage.

The Bottom Line

We have been very happy with the product, and have continued to use and purchase the product through PBL Assay Science.

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