Invitrogen by Thermo Fisher Scientific
anti-golgin 97
A-21270
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Browse Similar Products: Golgin-97 Immunofluorescence Antibodies
This antibody was used to perform immunofluorescence microscopy on HeLa cells transfected with an ER-targeted mClover construct. The antibody was specific and showed clear immunoreactive staining that had partial co-localization with the endoplasmic reticulum. Staining was discreet, clear and as predicted
Immunofluorescence
HeLa Cells
Anti-golgin 97 at 1:300 in blocking agent for 1 hour at room temperature
PBS with 1% normal goat serum and 0.1% triton-x 100
Goat anti-mouse conjugated to Alexa 568 for 1 hour room temp at 1:300
ER-targeted mClover transfection
Confocal microscopy
Staining was strong with very little background using HeLa cells. Golgi appeared near the nucleus in association with the endoplasmic reticulum.
N/A
Specific staining
The antibody can be a little expensive
This antibody is an excellent choice for immunofluorescence microscopy. It is specific with strong immunoreactivity.