Human CD158 Antibody

Genetics
Albert Einstein College of Medicine
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Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD158e/k (KIR3DL1/DL2)-PE-Vio770, human (clone: 5.133)

Catalog Number:

130-099-958

Our laboratory investigates different ways to improve immune responses against HIV, including enhancement of NK mediated ADCC activity. We tested this antibody to use for staining of NK cells as CD158 is mostly expressed on NK cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:10, 10min, 4C

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

Good staining of CD158 positive cells

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Works well

The Bad

None

The Bottom Line

The antibody works well but we ultimately decided to just use CD16 and CD56 staining to detect NK cells.

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