The Good
Excellent separation between positive and negative populations. Low background staining
The Bad
Significant overflow into the neighboring BV605/SB600 channel.
The Bottom Line
Excellent choice for the BV510 fluorochrome. When designing staining panel, ensure that antibody used in BV605/SB600 channel is not co-expressed on CD8+ T cells to minimize the impact of the significant spillover from BV510.