Peroxidase Kit For ELISA

University of Maryland, College Park
Cell Biology and Molecular Genetics
Post-doctoral researcher

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Company:

BIO-RAD

Product Name:

TMB Peroxidase EIA Substrate Kit

Catalog Number:

1721067

CD4+ T cell function is one focus of our laboratory. We commonly utilize ELISAs to measure/detect cytokine levels in cell culture supernatants. We use the BIO-RAD TMB Peroxidase EIA Substrate Kit to develop our ELISAs. The kit is very reliable and delivers repeatable results.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Cell culture supernatants

Protocol Overview

Perform ELISA as normal. Following incubation with secondary, add solution A and B according to the manufacturer's instructions to develop ELISA. Read plates with an ELISA reader.

Tips

Optimize sample dilution before performing ELISA

Results Summary

Antigens are detected readily with little background signal

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Can be used in in-house ELISAs

The Bad

None

The Bottom Line

Great kit for use in ELISAs

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