LI-COR
REVERT™ Total Protein Stain Kit
926-11010
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Normalization of western blot loading is critical when assessing protein levels in a given sample. LI-COR offers a water-soluble fluorescent stain to quantify total loaded protein for western blotting. Following transfer, blots are stained with the kit and total protein can be imaged on a LI-COR western blotting machine. The dye can then be washed out and blot can be blocked and put in primary. Total protein for each sample on the blot can be quantitated using LI-COR imaging software.
Western blot total protein quantitation for normalization
REVERT™ Total Protein Stain Kit and blot
Rinse nitrocellulose membrane after transfer with water, incubate membrane in 5mL Total Protein Stain for 5min with gentle shaking, drain stain and wash membrane twice in wash solution, image membrane in 700 channel on imaging system, block membrane and blot as usual
Be sure to thoroughly wash membranes before moving on to blocking step
Total protein can be visualized in much the same manner as performing a Ponceau stain
N/A
No need for additional incubations with loading control antibodies such as GAPDH or Actin
None
Great alternative for assessing protein loading when doing western blot without the need for additional antibody incubations
Product Name: REVERT ™ Total Protein Stain KitSupplier Page