Rabbit Akt3 mAb for Western Blot

Pathology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Akt3 (62A8) Rabbit mAb

Catalog Number:

3788

Our lab studies the role of Akt3 in various mouse models used to study Multiple Sclerosis. We have utilized many different anti-Akt3 antibodies for western blot and IP.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse brain homogenate

Primary Incubation

Akt3 (62A8) Rabbit mAb

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

LI-COR anti-rabbit IRDye secondary antibody

Tertiary Incubation

None

Detection

LI-COR Odyssey

Results Summary

The antibody works well for western blot with little to no background signal and a single band at the expected molecular weight (~60kDa).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background, single band at expected molecular weight

The Bad

Rabbit "mAb"...if such a thing actually exists.

The Bottom Line

Great antibody for western blots for Akt3 in mouse

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