Aves Labs Chicken anti-GFP

April 05, 2017

BlueskyReddit
Dominick P. Purpura Department of Neuroscience
Albert Einstein College of Medicine
Graduate student

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Company:

Aves Labs, Inc

Product Name:

Green Fluorescent Protein (GFP) Antibody

Catalog Number:

GFP-1010

Browse Similar Products: GFP Immunofluorescence Antibodies

The goal of these experiments was to detect GFP expressed by an adeno-associated viral vector. This product was selected because it could be used in combination with antibodies to detect other proteins that were grown in other species (mouse, rabbit, etc.). Frequently, we wanted to observe GFP expressing cells or fibers in an area known to express another protein, i.e. tyrosine hydroxylase.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Sections from mouse cryopreserved brains, collected directly onto slides

Primary Incubation

1:1000

Blocking Agent

5% NGS/0.3% TritonX-100/PBS

Secondary Incubation

1:1500 Alexa 488

Tertiary Incubation

NA

Detection

Zeiss fluorescence microscope

Results Summary

Images were taken of antibody-stained sections from cryopreserved brains. The goal was to observe colabeling of GFP-positive fibers in an area of the brain also expressing tyrosine hydroxylase. The amount of fluorescence was not quantified.

DOI or PMID #

NA

Additional Notes

NA

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Summary

The Good

Sections from cryopreserved brains and collected directly onto slides and stained; floating sections are not necessary for sufficient labeling; antibody is strong, large volumes are not needed, lasts a while

The Bad

None

The Bottom Line

Having a chicken antibody for GFP is good because it can co-incubated with antibodies from other species. This has been really useful to our lab.

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