Galanin in Human Tissue

Toxicology
University of Kentucky
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Galanin Antibody

Catalog Number:

NBP2-33582

We wanted to test the levels of certain neuropeptides in tissue. We used human brain sections (FFPE) for optimization. It was not easy finding antibodies that have been verified for IHC. We chose this antibody by a process of elimination.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human hippocampus

Primary Incubation

1 in 500. Overnight @ 4 degrees celsius.

Blocking Agent

ACUITYAdvanced Reagent 1 (Serum Block)

Secondary Incubation

ACUITYAdvanced Reagent 2 (Boost)

Tertiary Incubation

ACUITYAdvanced Reagent 3 (HRP Polymer)

Detection

DAB/metal concentrate

Results Summary

We found positive staining in the ependymal cells of the choroid plexus. This was not totally expected, but it was clear and consistent. We don't think it's non-specific as the stroma shows no brown coloration.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong staining at low antibody concentration

The Bad

The company's images are not helpful

The Bottom Line

We think it's a decent antibody, and we will use it in our upcoming experiments.

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