Great Product For Reverse Transcription For PCR

Albert Einstein College of Medicine
Pathology
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BIO-RAD

Product Name:

iScript™ cDNA Synthesis Kit

Catalog Number:

1708890

Our lab routinely performs real-time RT-PCR which requires cDNA synthesis. This product allows a wide range of RNA input concentrations which yields highly reproducible results.

Experimental Design and Results Summary

Application

Reverse Transciption

Starting Material

RNA

Protocol Overview

Extract RNA via desired extraction protocol, mix cDNA master mix, add RNA, reverse transcribe

Tips

Load as much RNA as possible (up to 1ug) and dilute resulting cDNA to make reactions last long (more PCR reactions)

Results Summary

High quality cDNA for downstream applications, mainly real-time RT-PCR

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Wide RNA input range

The Bad

May not be as cheap as other competitors

The Bottom Line

Great reagent for cDNA synthesis

Join the discussion