Arginase-1 Rabbit mAb

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Arginase-1 (D4E3M™) XP® Rabbit mAb #

Catalog Number:

93668

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue homogenates

Primary Incubation

1:1000 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

1:3000 at room temperature for 2 hour in 5% skimmed milk

Tertiary Incubation

NA

Detection

Western Blotting

Results Summary

Nice Arginase-1 was detected in mouse tissue homogenates.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good signal with primary antibody

The Bad

low degree of background

The Bottom Line

Efficient detection of Arginase-1, with low background.

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