Cell Signaling Technology
TNF-α (D2D4) XP® Rabbit mAb (Mouse Specific)
11948
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.
Western Blot
Mouse tissue homogenates
1:1000 at 4 degrees for overnight incubation
5% skimmed milk in 1X TBST
1:3000 at room temperature for 2 hour in 5% skimmed milk
NA
Western Blotting
Decent TNF-alpha signals were detected.
Good primary antibody, no background
None
Efficient detection of TNF-alpha, with minimal background.