Invitrogen by Thermo Fisher Scientific
IL-22 Polyclonal Antibody
PA5-34944
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.
Western Blot
Mouse tissue homogenates
1:10,000 at 4 degrees for overnight incubation
5% skimmed milk in 1X TBST
1:3000 at room temperature for 2 hour in 5% skimmed milk
NA
Western Blotting
Decent amounts of IL-22 were detected in mouse tissue homogenates.
Good signal with primary antibody
None
Efficient detection of IL-22, with minimal background.