Invitrogen by Thermo Fisher Scientific
IL-5 Monoclonal Antibody (TRFK5)
MM550C
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.
Western Blot
Mouse tissue homogenates
1:1000 at 4 degrees for overnight incubation
5% skimmed milk in 1X TBST
1:2500 at room temperature for 2 hour
NA
Western Blotting
IL-5 was detected in mouse tissue homogenates.
Good signal with primary antibody
None
Good signal with primary antibody, specific and little background.