Cell Signaling Technology
IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific)
12912S
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.
Western Blot
Mouse whole cell lysates
1:1000 at 4 degrees for overnight incubation
5% skimmed milk in 1X TBST
1:3000 at room temperature for 2 hour in 5% skimmed milk
NA
Western Blotting
Whole cell lysates showed presence of IL-6 protein upon LPS stimulation to the cells and levels were decreased upon addition of inhibitor.
Good signal with primary antibody, specific and no background
None
Efficient detection of IL-6, with minimal background.