GAPDH Antibody

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Fitzgerald

Product Name:

GAPDH antibody

Catalog Number:

10R-G109a

This mouse monoclonal antibody is ideal for use as a loading control in Western Blot studies. This widely published antibody will allow visualization of crisp bands on the membranes at low dilution. We have tried other GAPDH antibodies and this works the best.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat Tissue Lysate

Primary Incubation

1:25000 for 1 hour

Blocking Agent

5% milk in TBS-T

Secondary Incubation

1:5000 at room temperature for 1 hour

Tertiary Incubation

None

Detection

SuperSignal West Femto Maximum Sensitivity Substrate

Results Summary

We see sharp clear, crisp bands which can pick up even low levels of protein. We highly recommend using this first.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great binding affinity at low concentration.

The Bad

Nothing

The Bottom Line

Great GAPDH binding antibody.

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