Invitrogen by Thermo Fisher Scientific
AlamarBlue® Cell Viability Reagent
DAL1025
Info:Supplier Page View Product Specs View Product
We use this reagent in cytotoxicity assays where we test different antibody-drug conjugates for their ability to specifically kill HIV infected cells.
Cytotoxicity assay
Different treatment groups of activated ACH2 cells
At endpoint of cytotoxicity experiment add the alamar blue reagent directly to the wells containing cells. Incubate the plate(s) at 37C until colorimetric change is visible in wells that are a positive control for viable cells (incubation time can depend on the cell types used). Read fluorescence or absorbance on the appropriate plate reader.
If using adherent cells can wash the cells and re-do the experiment. This is useful if you perhaps let the reaction go on for too long and you can not detect a difference between different treatment groups.
Easy to read results, easy analysis. With some optimization of incubation time you can achieve very clean data of cell viability.
N/A
Works well
Some optimization required
Would recommend this product
Product Name: AlamarBlue Cell Viability ReagentSupplier Page