Anti Human CD4 Antibody

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-human CD4 Antibody

Catalog Number:

317427

Our lab has developed different mouse models to study different aspects of HIV infection. One of the models we have generated consists of injecting HIV-infected human PBMC into the spleens of NSG mice. In order to validate the presence of the human cells and all the subpopulations in the spleen, we perform FACS analyses at the experimental endpoints. Human CD4 antibody allows us to distinguish the human CD4+ T cells in the mouse spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Humanized mouse spleen cells

Primary Incubation

1:100, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good staining for CD4+ T cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining, easy optimization

The Bad

None

The Bottom Line

Would recommend this antibody

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