Anti Human CD3 Antibody

March 21, 2017

BlueskyReddit
Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Human CD3

Catalog Number:

555339

Our lab works with different mouse models to study different aspects of HIV infection. One of the models we have generated consists of injecting HIV-infected human PBMC into the spleens of NSG mice. In order to validate the presence of human cells and all the subpopulations in the spleen, we perform FACS analyses at the experimental endpoints. Human CD3 antibody is used to distinguish the human T cell population in the mouse spleen,

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Humanized mouse spleen cells

Primary Incubation

1:100, 30min 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Great staining for human CD3+ cells, clean population, no unspecific staining

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

None

The Bottom Line

Would recommend this antibody

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