CD117 Antibody for Flow cytometry

PRIMATE RESEARCH CENTER
UNIVERSITY OF WISCONSIN
Scientist I

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Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CD117 antibody

Catalog Number:

313206

NHP iPSCs were differentiation into hematopoietic progenitors using OP9 system. CD117 antibody was used to detect the expression of c-Kit in the cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Hematopoietic progenitors derived from NHP iPSCs

Primary Incubation

3ul of antibody for 100ul of cells in FACS buffer for 20 mins at RT

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

MAQSQuant

Results Summary

The antibody showed a clean seperation of c-Kit positive cells within the CD45+ population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good separation

The Bad

None

The Bottom Line

The antibody can be used efficiently to detect c-Kit positive cells.

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