Quality L-Selectin Antibody

March 17, 2017

Microbiology
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD62L Antibody

Catalog Number:

104412

We often perform quick characterizations of T cell phenotypes following virus infection. We use this antibody in combination with CD44 to roughly define T cell phenotypes. BioLegend is our go-to company for most flow cytometry reagents, as they have a vast array of quality products.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:400 - 25 minutes on ice

Blocking Agent

FcR blocking antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

This antibody allows for the separation of distinct CD62L-high and CD62L-low populations by flow cytometry. Signal is always strong when staining splenocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and easy to use

The Bad

n/a

The Bottom Line

Great antibody for rough characterization of splenic T cells

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