Enhanced Chemiluminescent Substrate For Western Blot Analysis

Albert Einstein College of Medicine
Physiology and Biophysics
Graduate student

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Company:

ThermoFisher Scientific

Product Name:

SuperSignal™ West Femto Maximum Sensitivity Substrate

Catalog Number:

34095

Substrate for chemiluminescent detection of secondary antibodies conjugated with horseradish peroxidase (HRP) enzyme in Western blot analysis

Experimental Design and Results Summary

Application

Western blot

Starting Material

Membrane probed with target primary and HRP-conjugated secondary antibodies

Protocol Overview

Mix solutions at 1:1 ratio, incubate blot with mixture and image using either film or imaging system.

Tips

Incubate for at least 5 minutes

Results Summary

Clear chemiluminescent signal is detected

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Low anounts of protein can be detected using this kit

The Bad

Need refrigeration

The Bottom Line

Great substrate for HRP ECL detection

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