Anti-CEP192 Immunofluorescence Antibody

March 15, 2017

BlueskyReddit
Physiology and Biophysics
Albert Einstein College of Medicine
Graduate Student

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Company:

Sigma Aldrich

Product Name:

Anti-CEP192 Antibody (produced in rabbit)

Catalog Number:

HPA040503

Browse Similar Products: CEP192 Immunofluorescence Antibodies

This rabbit polyclonal antibody to Cep192 did not bind to the centrosome of methanol fixed H460 cells. We had hoped to use this to compare centrosomal fluorescence intensity, but were unable to to the lack of binding.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Methanol fixed H460 cells

Primary Incubation

1:500 at room temperature for 1.5 hours

Blocking Agent

5% NGS

Secondary Incubation

1:750 rhomadine conjugated anti-rabbit antibody

Tertiary Incubation

None

Detection

Confocal Microscope

Results Summary

The antibody did not bind to Cep192's known centrosomal localization.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Shown to work elsewhere in IHC

The Bad

Doesn't work for immunofluorescence.

The Bottom Line

Do not use if you are planning immunofluorescence experiments.

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