Sigma Aldrich
Monoclonal ANTI-FLAG M2 antibody
F1804
Browse Similar Products: FLAG M2 Western Blot Antibodies
This mouse monoclonal antibody is ideal for Western Blotting applications. We used lysate from cells expressing recombinant FLAG tagged antibody and were able to see clear bands. This antibody is widely published.
Western Blot
U2OS lysate from cells expressing recombinant FLAG tagged protein
1:100 at 4 degrees overnight
5% milk in TBS-T
1:5000 at room temperature for 1 hour
None
1:5000 anti-mouse HRP antibody
Following induction of a recombinant protein cells were harvested. FLAG protein was blotted for with 1:100 M2 antibody in 5% milk in TBS-T. Following three 5 minute washes, the membranes were probed with HRP secondary at 1:5000 at room temperature for 1 hour.
N/A
Strong binding with minimal background
Antibody dilution is 1:100 so you use it quickly.
Great antibody.