Cell Signaling Technology
Phospho-Stat3 (Tyr705)
9131
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This antibody can be used to detect the canonical form of activated STAT3. STAT3 is activated by the phosphorylation of tyrosine residue 705
Western Blot
HUVEC lysate
1:2000, overnight
5% BSA
1:5000, 1 hour
NA
ChemiDoc™ Touch Imaging System
Clean bands of pSTAT3705 were detected
N/A
Primary incubation in BSA, NOT milk
Clean band
Relatively high background.
A good antibody to detect pSTAT3Y705 by Western blot