Abcam
Src antibody
ab32102
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To test the stability of protein targets implicated in serum starvation, Hep G2 cells were used to perform the pulse-chase experiments by Western blotting
Western Blot
Hep G2
1/1000
5% milk
1 hour room temperature
The enhanced chemiluminescence kit
The expression levels of Src remained unchanged with time elapse after cycloheximide stimulation
N/A
The antibody works
The system worked