Laminin Alpha 2 Antibody

February 23, 2017

Urology
Children's Hospital Los Angeles
Research Specialist II

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Laminin 2 alpha antibody [4H8-2]

Catalog Number:

ab11576

Laminin 2 alpha antibody is an extracellular protein that plays an important role in the kidney's basement membrane. This antibody helps to identify protein that leads to abnormalities with the thickening or thinning of the basal membrane which can lead to renal disease progression.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse Kidney

Primary Incubation

[1:100] Overnight at 4C

Blocking Agent

BSA 5%

Secondary Incubation

Donkey anti-Rat Alexa Fluor 488

Tertiary Incubation

N/A

Detection

Microscopy

Results Summary

Staining works better if left incubating overnight at 4C. However, it does need several washes but the signal is strong and clear.

DOI or PMID #

N/A

Additional Notes

Works best with High pH Citrate Buffer Antigen Retrieval

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Summary

The Good

Signal is strong and clear

The Bad

Need to leave it overnight for best results. Also, need to wash it a few times to remove debris.

The Bottom Line

This is a quick and efficient way to identify laminin proteins. Antibody works well, but there is a lot of debris, so extra washes are necessary.

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