WGA Antibody

Urology
Children's Hospital Los Angeles
Research Specialist II

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Wheat Germ Agglutinin antibody (FITC)

Catalog Number:

ab20528

Aim of our research is to study kidney progenitor cells and how they can decelerate chronic kidney disease. This antibody was used to see kidney connective tissue to compare diseased kidney and kidney treated with progenitor cells. This antibody is efficient, quick staining and great results.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse Kidney

Primary Incubation

1 Hour,(1:1000) concentration

Blocking Agent

5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Microscopy

Results Summary

The antibody was tested with Frozen and Paraffin slides. The antibody does not work with Paraffin slides, but it works really well in frozen sections. The signal is strong and the staining is evenly consistent!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works really well!

The Bad

Only works in Frozen sections

The Bottom Line

Only works well in Frozen sections, Fixed in 10% Formalin.

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