Anti Human CD8 APC-Cy7

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC/Cy7 anti-human CD8a Antibody

Catalog Number:

301016

We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by activating them via TCR specific MHC-peptide ligands, and co-stimulatory molecules. We use purified CD8 T cells from naive donors. We do different FACS experiments with these CD8+ T cells, including staining for activation markers and cytokines after co-culturing the CTLs with cells presenting peptides specific for the TCRs expressed by the CTLs. In all of these experiments we stain for hCD8 so that we can gate on the CTLs specifically.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Purified human CD8 T cells

Primary Incubation

1:100, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good staining for human CD8, minimal background on negative samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

None

The Bottom Line

Would recommend

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