Works Well To Remove rRNA Before Sequencing

UCSF
Urology
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Illumina

Product Name:

Ribo-Zero Gold rRNA removal (Human/Mouse/Rat)

Catalog Number:

MRZG126

I wanted to sequence total RNA from mouse cells so I wanted to remove ribosomal RNA. This kit was easy to use and gave great results.

Experimental Design and Results Summary

Application

Remove rRNA before RNA-Seq

Starting Material

Several types of mouse cells

Protocol Overview

First you heat and then cool your RNA and probe mixture to bind rRNA to their probes. Then you put on magnetic beads that pull the probes out of the solution.

Tips

Max out the RNA input

Results Summary

Works well, usually left with between 4-6% rRNA after sequencing.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works well

The Bad

Expensive

The Bottom Line

If you want to remove rRNA before sequencing there aren't many cheap options, this kit at least works well and is easy to use.

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