Biolegend
anti-mouse F4/80 FITC
123108
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This antibody was used as a marker for monocytes/macrophages in mouse splenocytes. It was chosen because it is a commonly used clone for F4/80, FITC works well with other fluorophores in the staining panel, and Biolegend had a great price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
F4/80 staining was great! Positive cells were fully resolved from negative.
Works well, low cost.
Will continue to use this antibody.