Staining PBMC With Anti-CD3 FITC

March 08, 2017

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

anti-human CD3 FITC

Catalog Number:

11-0038-42

This antibody was used as a marker for T cells in human blood. It was chosen because it is a commonly used clone for CD3, FITC works well with other fluorophores in the staining panel, and eBioscience had a great price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 15 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

CD3 staining was great! Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well, low cost.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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