Staining PBMC With Anti-CD19 PE

March 07, 2017

BlueskyReddit
Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-human CD19 PE

Catalog Number:

302207

Browse Similar Products: CD19 Flow Cytometry Antibodies

This antibody was used as a marker for B cells in human blood. It was chosen because it is a commonly used clone for CD19, PE is bright and works well with other fluorophores in the staining panel, and Biolegend had a great price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 15 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

CD19 staining was great! Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well, low cost.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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