Biolegend
anti-human TNFa PE
502908
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This antibody was used to measure TNFa production in CD8+ T cells. It was chosen because it is a commonly used clone for TNFa, PE is bright and works well with other fluorophores in the staining panel, and Biolegend had a great price.
Flow Cytometry
Human T cells
5uL per test in 100uL total staining volume for 15 minutes at room temperature
2% FBS
N/A
TNFa staining was great! Positive cells were fully resolved from negative.
Works well, low cost.
Will continue to use this antibody.