Staining T Cells With Anti-CD4 BV421

February 28, 2017

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-human CD4 BV421

Catalog Number:

317433

This antibody was used to distinguish CD4+ T cells from CD8+ T cells. It was chosen because it is a commonly used clone for CD4, BV421 is bright and works well with other fluorophores in the staining panel, and Biolegend had a decent price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T cells

Primary Incubation

5uL per test in 100uL total staining volume for 15 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

CD4 staining was as expected. Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

Somewhat expensive

The Bottom Line

Will continue to use this antibody.

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